Harrie Smolders clinched victory with his anchorman ride aboard Monaco, while Team Ireland finished a very close second.
"I try to protect him now and just really aim for the classes that matter," said Tiffany of the 15-year-old gelding winning the final grand prix of the season at Tbird.
France finished second with 10 faults, Norway was third with 21 and the Swiss slotted into fourth with an uncharacteristic 25.
The Canadian rider steps up from a win in the U25 last week to record her first senior FEI victory in the $36,900 CSI2* 1.45m class.
Team Germany punched their ticket to Sunday's deciding final; the Spanish were impressive in front of their home crowd in Barcelona.
Vanessa Mannix (CAN) finished second with Balzane du Mazes, while Brennan's fiancée Lauren Crooks (USA) finished third.
Belgium, Brazil, Canada, France, Great Britain, Germany, Ireland, Italy, Netherlands, Norway, Spain, Sweden, Switzerland, USA and Uzbekistan will compete.
A previous series winner, she reclaimed the title with Havana, a horse she has developed herself since the mare was four years old.
The Irishman kicked off the North American League with a convincing victory aboard Vital Chance de la Roque, edging Canada's Vanessa Mannix.
The pair lead wire-to-wire in the $15,000 CSI4*-W 1.45m at Thunderbird Show Park's Harvest Celebration over Justin Prather and EZ Ace.